5-Propargylamino-dCTP-Cy5

SKU: orb1733849

Description

5-Propargylamino-dCTP-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy5/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy5/70-50% dCTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1159.01 g/mol (free acid); Detected MW: 1158.23 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₅H₅₇N₆O₂₀P₃S₂
• SMILES: N(CC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@H](C1)O)C(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=C\C=C\C=C\C1=*(c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only