5-Propargylamino-dCTP-Cy3

• Catalog Number: orb533140
• Category: Tools
• Description: 5-Propargylamino-dCTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy3/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy3/70-50% dCTP.
• Concentration: 1.0 mM-1.1 mM
• Form/Appearance: solution in 10 mM Tris-HCl; Color: pink; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1132.97 g/mol (free acid); Detected MW: 1132.21 g/mol (free acid)
• SMILES: N(CC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@H](C1)O)C(=O)CCCCCN1c2ccc(S(=O)(=O)[O-])cc2C(C)(C)/C/1=C\C=C\C1=*(c2ccc(cc2C1(C)C)S(=O)(=O)O)CC
• Formula: C₄₃H₅₅N₆O₂₀P₃S₂
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by: Primer Extension with Klenow fragment; PCR with Taqpolymerase in-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. < br>< b>Spectroscopic Propertie: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.