5-Propargylamino-dCTP-ATTO-647N

SKU: orb1733853

Description

5-Propargylamino-dCTP-ATTO-647N is recommended for direct enzy-matic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-647N/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-647N/70-50% dCTP.

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1148.08 g/mol (free acid); Detected MW: 1147.40 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₅₄H₆₈N₇O₁₅P₃
• SMILES: [C@@H]1(CC(OC1COP(=O)(OP(=O)(OP(=O)([O-])O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCN(C(=O)c1c(C2=c3cc4c5=[N+](CCC4)CCCc5c3C(c3c2cc2c(c3)N(C(CC2C)(C)C)CC)(C)C)cccc1)C)N)O

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only