5-Propargylamino-dCTP-ATTO-550

SKU: orb1733854

Description

5-Propargylamino-dCTP-ATTO-550 is recommended for direct enzy-matic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-550/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-550/70-50% dCTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by-Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 554 nm, λem 576 nm, ε 120.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1095.97 g/mol (free acid); Detected MW: 1095.33 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₉H₆₀N₇O₁₆P₃
• SMILES: [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)([O-])O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCN(C(=O)c1c(C2=c3cc4C(CC([NH+]=c4cc3Oc3cc4NC(CC(c4cc23)C)(C)C)(C)C)C)cccc1)C)N)O

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only