2-Bromopalmitic acid

SKU: orb1304098

Description

2-Bromohexadecanoic acid (2-BP) is a synthetic palmitate analog that functions as a PPARδ agonist and a potent inhibitor of protein palmitoylation by targeting DHHC palmitoyltransferases. This non-metabolizable compound is widely used in vitro and in vivo to study lipid metabolism, signal transduction, and the role of palmitoylation in diseases.

Research Area

Cell Biology, Immunology & Inflammation

Key Properties

• CAS Number: 18263-25-7
• MW: 335.23
• Purity: >98%
• Formula: C₁₆H₃₁BrO₂
• SMILES: CCCCCCCCCCCCCCC(Br)C(O)=O
• Target: Pyroptosis,Others
• Solubility: Soluble in DMSO (up to 75 mg/ml) or in Ethanol (up to 25 mg/ml).

Bioactivity

• Target IC50:
  UNC-63 (C. elegans):1.6 ± 0.7 μM|multiple palmitoyl-acyl transferase (PAT) activity towaRD cellss FarnCNRas (NBD):4 µM|multiple palmitoyl-acyl transferase (PAT) activity towaRD cells MyrGCK (NBD):4 µM
• In Vitro:
  METHODS: HepG2 was treated with PA (200 µM) and 2-Bromohexadecanoic acid (25-150 µM) to examine CSC sphere morphology and number. RESULTS: 2-Bromohexadecanoic acid (25, 50 and 150 µM) significantly reduced PA-induced CSC sphere formation in HepG2 (75-150 µM and >150 µM.) METHODS: ERα or ERα-Cys447Ala-transfected HeLa and HepG2 cells were pretreated with 2-Bromohexadecanoic acid (10 µM) for 30 min, then stimulated with E2 (10 nM) for 10 min, and the target protein expression levels were detected by Western Blot. RESULTS: E2 induced ERK and AKT phosphorylation in ERα-transfected HeLa and HepG2 cells. E2-induced signaling kinase activation was completely blocked without affecting the basal level of phosphorylation if the cells were pretreated with the PAT inhibitor 2-Bromohexadecanoic acid.E2 was ineffective in inducing phosphorylation of ERK and AKT in ERα-Cys447Ala mutant-transfected HeLa cells.

Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2-BP

Compound Identifiers

PubChem CID

82145