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Human IL3 protein
Catalog Number: orb80057
| Catalog Number | orb80057 |
|---|---|
| Category | Proteins |
| Description | Human IL3 Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL3 is purified by proprietary chromatographic techniques. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | IL3 |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin-3 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is 0.1 ng/ml, corresponding to Specific Activity of 10,000,000 IU/mg. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | Lyophilized from concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3. |
| Alternative names | MCGF (Mast cell growth factor) protein, Multi-CSF Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-3 as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL3 protein
- Item 1 of 1
Human IL3 protein (Active) [orb358969]
> 96% as determined by SDS-PAGE and HPLC.
15.0 kDa
E.Coli
500 μg, 10 μg, 100 μg - Item 1 of 1
Human IL3 protein [orb594806]
Greater than 95% as determined by SDS-PAGE.
16.1 kDa
Mammalian cell
1 mg, 500 μg, 10 μg, 50 μg - Item 1 of 1
Human IL3 protein [orb594807]
Greater than 95% as determined by SDS-PAGE.
16.6 kDa
E.coli
1 mg, 500 μg, 10 μg, 50 μg - Item 1 of 1
Human IL-3 Protein, His Tag [orb1087548]
Unconjugated
90%
17.0 kDa
Human IL-3 Protein, His Tag, premium grade (orb1087548) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1).
20 μg, 1 mg, 100 μg - Item 1 of 1
Mouse IL-3 Protein, His Tag [orb1101195]
Unconjugated
90%
17.6 kDa
Mouse IL-3 Protein, His Tag (orb1101195) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Cys 166 (Accession # P01586-1).
1 mg, 100 μg
