You have no items in your shopping cart.
You have no items in your shopping cart.

| Catalog Number | orb391417 |
|---|---|
| Category | Proteins |
| Description | Recombinant human CD47 protein is produced by our mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a 6His tag at the C-terminus. |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PVDHHHHHH |
| UniProt ID | Q08722 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | Human cells |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | MER6; Leukocyte surface antigen CD47; Antigenic su Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 961 |
| Expiration Date | 6 months from date of receipt. |
Human | |
0.32-20 ng/mL | |
0.111 ng/mL |
Human | |
0.16-10ng/mL | |
0.1 ng/mL |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide. | |
Mammalian |
Biotin | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 16.4 KDa. Observed: 30-40 KDa, reducing conditions |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 40.3 KDa. Observed: 48-65 KDa, reducing conditions |
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a Review