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Catalog Number | orb348813 |
---|---|
Category | Proteins |
Description | T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
Reactivity | Human |
Tag | C-Fc & C-6×His | C-Fc & C-Flag |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 39.2 kDa (CD3E) & 38.6 kDa (CD3G) |
Target | CD3E & CD3G |
Protein Sequence | NP_000724.1 (CD3E) & AAI13831.1 (CD3G) |
Source | Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (orb348813) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)). |
Expression System | HEK293 |
Expression Region | Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) |
Endotoxins | 1.0 EU per μg |
NCBI | CD3, AAI13831.1, NP_000724.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CD3 epsilon & CD3 gamma,CD3E & CD3G Read more... |
Note | For research use only |
Application notes | Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD3 epsilon & CD3 gamma Heterodimer protein
Immobilized OKT3 MAb at 10ug/mL (100 uL/well) can bind Human CD3E & CD3G with a linear range of 0.3-5 ug/mL.
Unconjugated | |
90% | |
16.9 kDa | |
Human CD3 epsilon, His Tag (orb257326) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1). |
Unconjugated | |
95% | |
39.2 kDa | |
Human CD3 epsilon, Fc,His Tag (orb257328) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1). |
Greater than 90% as determined by SDS-PAGE. | |
47.7 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
15.8 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
13.8 kDa | |
Yeast |
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