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Catalog Number | orb257326 |
---|---|
Category | Proteins |
Description | CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 16.9 kDa |
Target | CD3 epsilon |
Protein Sequence | NP_000724.1 |
Source | Human CD3 epsilon, His Tag (orb257326) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1). |
Expression System | HEK293 |
Expression Region | Asp 23 - Asp 126 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000724.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | FLJ18683,T3E,TCRE,CD3E,CD3-epsilon Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD3 epsilon protein
Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 ug/mL (100 uL/well) can bind Human CD3E, His Tag with a linear range of 0.4-6.25 ng/mL.
Unconjugated | |
95% | |
39.2 kDa (CD3E) & 38.6 kDa (CD3G) | |
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (orb348813) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1 (CD3E) & P09693-1 (CD3G)). |
Unconjugated | |
95% | |
39.2 kDa | |
Human CD3 epsilon, Fc,His Tag (orb257328) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1). |
Greater than 90% as determined by SDS-PAGE. | |
47.7 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
15.8 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
13.8 kDa | |
Yeast |
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