Human CD3 epsilon Protein

• Catalog Number: orb257328
• Category: Proteins
• Description: CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
• Reactivity: Human
• Tag: C-Fc & C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 39.2 kDa
• Target: CD3 epsilon
• Protein Sequence: NP_000724.1
• Source: Human CD3 epsilon, Fc,His Tag (orb257328) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).
• Expression System: HEK293
• Expression Region: Asp 23 - Asp 126
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000724.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 39.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human CD3 epsilon protein