HDAC1 Antibody

• Catalog Number: orb2635064
• Category: Antibodies
• Description: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/ CBP-associated factor), p300/CBP and the TFIID subunit TAFII p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-HDAC1-1B7
• Tested applications: ELISA, FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: Recombinant full-length human protein was used as the immunogen for the HDAC1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HDAC1 antibody is available for research use only.
• UniProt ID: Q13547
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the HDAC1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using HDAC1 antibody (clone PCRP-HDAC1-1B7). These results demonstrate the foremost specificity of the PCRP-HDAC1-1B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunofluorescent staining of PFA-fixed human HeLa cells using HDAC1 antibody (green, clone PCRP-HDAC1-1B7) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with HDAC1 antibody (blue, clone PCRP-HDAC1-1B7) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free HDAC1 antibody (clone PCRP-HDAC1-1B7) as confirmation of integrity and purity.