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H3K27me3 antibody

H3K27me3 antibody

Catalog Number: orb758980

DispatchUsually dispatched within 5-10 working days
$ 531.00
Catalog Numberorb758980
CategoryAntibodies
DescriptionMouse monoclonal antibody to H3K27me3
Species/HostMouse
ClonalityMonoclonal
Clone NumberBT164
Tested applicationsELISA, ICC, IF, IHC, IP, WB
ReactivityHuman
IsotypeIgG2b
ImmunogenBT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin.
Concentration1 mg/ml
PurityPurified
ConjugationUnconjugated
TargetH3K27me3
StorageStore at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Alternative namestrimethyl-Histone H3 (Lys27); histone H3; H3K27me3
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NoteFor research use only
Application notesBT164 recognizes an apoptosis-induced trimethylation of K27 on N-terminal tail of histone H3 which is a target for autoantibodies in patients and mice with SLE (systemic lupus erythematosus) (van Bavel et al., 2011; pmid: 20699234). Consequently, this antibody is recommended for the detection and studying of SLE. ELISA studies demonstrated that BT164 exhibits preferential activity with the apoptotic nucleosomes in comparison with the control nucleosomes (van Bavel et al., 2011; pmid: 20699234). It can be used for the detection of apoptosis in general. Furthermore, this antibody was demonstrated to label cells with methylated histone-3 on K27 via immunofluorescence and it also immunoprecipitated chromatin with such a methylation profile, further demonstrating its ability to specifically recognize markers characteristic of SLE (van Bavel et al., 2011; pmid: 20699234).
Expiration Date12 months from date of receipt.
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