H3K27me3 Antibody

• Catalog Number: orb1259103
• Category: Antibodies
• Description: H3K27me3 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: H3K27me3
• UniProt ID: Q16695
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1259103) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25 (40x lens).

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (orb1259103) at dilution of 1:25. Blue: DAPI for nuclear staining.