H3K27me3 Antibody

• Catalog Number: orb1258185
• Category: Antibodies
• Description: H3K27me3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Other, Rat
• Isotype: IgG
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 17kDa
• Target: H3K27me3
• UniProt ID: Q16695
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (orb1258185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1258185).

Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (orb1258185). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red) (orb1258185) and BrdU antibody (Green) (A1482). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.