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    H3K27me3 Antibody

    Catalog Number: orb1258185

    DispatchUsually dispatched within 5-10 working days
    $ 895.00
    Catalog Numberorb1258185
    CategoryAntibodies
    DescriptionH3K27me3 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsChIP, IF, IHC, IP, WB
    ReactivityHuman, Mouse, Other, Rat
    IsotypeIgG
    ImmunogenA synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
    Concentrationbatch dependent
    Dilution rangeWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
    Form/AppearanceLiquid
    ConjugationUnconjugated
    MWObserved: 17kDa
    TargetH3K27me3
    UniProt IDQ16695
    StorageStore at -20˚°C. Avoid freeze / thaw cycles.
    Buffer/PreservativesLiquid
    Alternative namesH3F3A, H3t, H3.4, H3/g, H3FT
    Read more...
    NoteFor research use only
    Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
    Expiration Date12 months from date of receipt.
    H3K27me3 Antibody

    Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (orb1258185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

    H3K27me3 Antibody

    Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (orb1258185).

    H3K27me3 Antibody

    Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

    H3K27me3 Antibody

    Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

    H3K27me3 Antibody

    Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100 (40x lens).

    H3K27me3 Antibody

    Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (orb1258185). Blue: DAPI for nuclear staining.

    H3K27me3 Antibody

    Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red) (orb1258185) and BrdU antibody (Green) (A1482). Blue: DAPI for nuclear staining.

    H3K27me3 Antibody

    Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.

    H3K27me3 Antibody

    Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (orb1258185) at dilution of 1:100. Blue: DAPI for nuclear staining.

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