H3C1 Antibody

• Catalog Number: orb1564023
• Category: Antibodies
• Description: H3C1 Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: R01-8I4
• Tested applications: ChIP, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
• Concentration: 0.3 mg/ml
• Dilution range: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ChIP: 1/20
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Target: H3C1
• UniProt ID: P68431
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: H3K27me3; H3 histone; HIST1H3A; Histone cluster 1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H3 (tri methylLys27) in C6, Jurkat lysates using TriMethyl-Histone H3 (Lys27) antibody.

Immunocytochemistry analysis of TriMethyl-Histone H3 (Lys27) (green) in Hela using TriMethyl-Histone H3 (Lys27) antibody, and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TriMethyl-Histone H3 (Lys27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.