GPC3 Antibody

• Catalog Number: orb1252114
• Category: Antibodies
• Description: GPC3 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SGPN3-1
• Tested applications: IF, IHC-P
• Reactivity: Human, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Glypican-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GPC3
• UniProt ID: P51654
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7
• Note: For research use only
• Application notes: Immunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Glypican-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human hepatocellular carcinoma with Glypican-3 antibody (SGPN3-1).

IHC testing of FFPE rat lung with Glypican-3 antibody (SGPN3-1).