GPC3 Antibody

• Catalog Number: orb1263867
• Category: Antibodies
• Description: GPC3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human Glypican 3 (GPC3).
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 66 kDa
• Target: GPC3
• UniProt ID: P51654
• NCBI: P51654
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in mouse stomach tissue lysates (35 ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

Glypican 3 (GPC3) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.