GPC3 Antibody

• Catalog Number: orb1260150
• Category: Antibodies
• Description: GPC3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human GPC3 (NP_004475.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 66kDa
• Target: GPC3
• UniProt ID: P51654
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: GPC3, SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, G
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using GPC3 antibody (orb1260150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver using GPC3 antibody (orb1260150) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using GPC3 antibody (orb1260150) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using GPC3 antibody (orb1260150) at dilution of 1:100 (40x lens).