GFAP antibody

• Catalog Number: orb43799
• Category: Antibodies
• Description: Mouse Monoclonal to GFAP.
• Clonality: Monoclonal
• Clone Number: GF-01
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Feline, Human, Porcine
• Isotype: Mouse IgG1
• Immunogen: Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: human brain (cortex, cerebellum). The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues. Immunocytochemistry: Recommended dilution: 5-10 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: GFAP
• Entrez: 2670
• UniProt ID: P14136
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-GFAP antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: human brain (cortex, cerebellum). The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues. Immunocytochemistry: Recommended dilution: 5-10 μg/ml.
• Expiration Date: 12 months from date of receipt.

References

Sana, Jiri et al. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival Sci Rep, 8, 2836 (2018)

PubMed: 29434344

Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-GFAP (clone GF-01).

Immunohistochemistry staining of human brain cortex (paraffin-embedded sections) with anti-GFAP (clone GF-01).