GFAP Antibody

• Catalog Number: orb1259841
• Category: Antibodies
• Description: GFAP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 50kDa
• Target: GFAP
• UniProt ID: P14136
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Glial fibrillary acidic protein, GFAP, GFAP
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using GFAP antibody (orb1259841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody (orb1259841) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody (orb1259841) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-251MG cells using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse brain using GFAP antibody (orb1259841) at dilution of 1:100. Blue: DAPI for nuclear staining.