GFAP antibody

• Catalog Number: orb500829
• Category: Tools
• Description: GFAP antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GFAP (51-150/432 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:200-1000, IF=1:200-800, Flow-Cyt=1μg/Test, IHC-F=1:200-1000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: GFAP
• UniProt ID: P14136
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of RSC96(blue).Primary Antibody:Rabbit Anti- GFAP antibody; Dilution:1μg in 100 μL 1X PBS containing 0.5% BSA.

Flow cytometry of U87MG.Primary Antibody (green line): Rabbit Anti-GFAP antibody; Dilution:1ug/Test.

ICC analysis of U87MG cell using GFAP polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of human glioma using GFAP Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse brain using GFAP Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of rat brain using GFAP Polyclonal Antibody at 1:200 dilution.

Western blot:Cerebellum (Rat);Cerebellum (Mouse);Eye (Mouse);Primary:Anti-GFAP at 1:300 dilution.

Western blot:U251 Cell;Primary:Anti-GFAP at 1:300 dilution.