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Catalog Number | orb1294403 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to GFAP |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
Dilution range | Western Blot 1:1000-1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Frozen) 1:100 – 1:300 Immunohistochemistry (Paraffin) 1:100 – 1:300 |
Conjugation | Unconjugated |
Target | GFAP |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical of frozen sections analysis of mouse cerebellum.Green : GFAP : 1:200;Anti-rabbit 488 : 1:500.
Immunofluorescence:cells were stained with anti-GFAP antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical of frozen sections analysis of mouse cerebellum.Green : GFAP : 1:200;Anti-rabbit 488 : 1:500.
Immunofluorescent analysis of primary cortical neurons.Red : GFAP : 1:200;Blue : DAPI was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling GFAP antibody with orb1294403 at 1:100.
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling GFAP antibody with orb1294403 at 1:100.
Western blot analysis using Anti-GFAP antibody at 1:1000 dilution, lysates/proteins at 60 μg per lane.
Western blot analysis using Anti-GFAP antibody at 1:1000 dilution, lysates/proteins at 60 μg per lane.
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Bovine, Canine, Porcine, Rabbit, Sheep |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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