FOXA1 Antibody

• Catalog Number: orb639711
• Category: Antibodies
• Description: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1, and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FOXA1/1516
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant human partial protein (amino acids 372-472) was used as the immunogen for the FOXA1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This FOXA1 antibody is available for research use only.
• UniProt ID: P55317
• Storage: Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human PIN (Prostatic Intraepithelial Neopplasia) with FOXA1 antibody (clone FOXA1/1516). HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1516). HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1516). HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1516). HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free FOXA1 antibody (clone FOXA1/1516) as confirmation of integrity and purity.