CD45RA Antibody (Leukocyte marker)

• Catalog Number: orb1824325
• Category: Antibodies
• Description: CD45 is a family of single chain transmembrane glycoproteins, consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are rod shaped, heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1q31-32.CD45 is exclusively expressed in haematolymphoid cells. Almost all haematolymphoid cells, including precursor cells and mature B- and T-lymphocytes, granulocytes, monocytes/histiocytes and interdigitating reticulum cells and follicular dendritic cells express CD45. This protein is detected in the large majority of haematolymphoid neoplasms, i.e., leukemias and malignant lymphomas. Overall, about 90% of malignant lymphomas are CD45 positive.CD45 is an important marker in the primary tumor screening panel in order to identify haematolymphoid differentiation. Loss of CD45 in precursor B-cell neoplasms is a negative prognostic parameter.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PTPRC/4367R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1-600) from the human protein was used as the immunogen for the CD45RA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD45RA antibody is available for research use only.
• UniProt ID: P08575
• Storage: Aliquot the CD45RA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with CD45RA antibody (clone PTPRC/4367R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with CD45RA antibody (clone PTPRC/4367R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD45RA antibody (clone PTPRC/4367R) as confirmation of integrity and purity.