CD45 Antibody (Leukocyte marker)

• Catalog Number: orb2643717
• Category: Antibodies
• Description: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Human recombinant full-length CD45 protein was used as the immunogen for the recombinant CD45 antibody.
• UniProt ID: P08575
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rPTPRC/1460
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant CD45 antibody is available for research use only.
• Note: For research use only

Western blot testing of human spleen lysate with recombinant CD45R antibody (clone rPTPRC/1460). Expected molecular weight: 147-220 kDa depending on glycosylation level.

IHC staining of FFPE human tonsil with recombinant CD45R antibody (clone rPTPRC/1460). HIER: steam section in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.

Flow cytometry testing of PFA-fixed human Raji cells with recombinant CD45R antibody (clone rPTPRC/1460); Red=isotype control, Blue= recombinant CD45R antibody.

SDS-PAGE analysis of purified, BSA-free recombinant CD45 antibody (clone rPTPRC/1460) as confirmation of integrity and purity.