Apolipoprotein E antibody

• Catalog Number: orb19012
• Category: Antibodies
• Description: Goat polyclonal antibody to Apolipoprotein E
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.05-0.1 μg/ml
• Conjugation: Unconjugated
• MW: 36.2
• Target: APOE
• Entrez: 348
• Protein Sequence: VGTSAAPVPSDNH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Anti-AD2 antibody, Anti-Apo-E antibody, Anti-APOE
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows extra-cellular staining between hepatocytes. Recommended concentration, 2-4ug/ml.WB: Approx. 36kDa band observed in Human Brain lysates (calculated MW of 36.2kDa according to NP_000032.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19012 (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19012 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.