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Catalog Number | orb371720 |
---|---|
Category | Antibodies |
Description | Apolipoprotein E/APOE Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, WB |
Reactivity | Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived mouse Apolipoprotein E recombinant protein (Position: D55-Q294). Mouse Apolipoprotein E shares 75% and 94.6% amino acid (aa) sequence identity with human and rat Apolipoprotein E, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 35867 MW |
UniProt ID | P08226 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Apolipoprotein E;Apo-E;Apoe; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of Apolipoprotein E using anti-Apolipoprotein E antibody.Lane 1:mouse spleen tissue; 2:mouse kidney tissue.
IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody. Apolipoprotein E/APOE was detected in immunocytochemical section of HEPA1-6 cells.
IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody. Apolipoprotein E/APOE was detected in a paraffin-embedded section of mouse liver tissue.
IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody. Apolipoprotein E/APOE was detected in a paraffin-embedded section of rat liver tissue.
IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody. Apolipoprotein E was detected in a paraffin-embedded section of mouse liver tissue.
IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody. Apolipoprotein E was detected in a paraffin-embedded section of rat liver tissue.
ELISA, FC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
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