APOE Antibody

• Catalog Number: orb1239091
• Category: Antibodies
• Description: APOE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E.The immunogen is located within amino acids 220 - 270 of APO-E.
• Concentration: 1 mg/mL
• Dilution range: APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 35 kDa Observed: 34 kDa
• Target: APOE
• UniProt ID: P02649
• NCBI: NP_000032
• Storage: APO-E antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: APO-E Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: APO-E Antibody: AD2, LPG, LDLCQ5, Apolipoprotein E
• Note: For research use only
• Application notes: APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 µg/mL.

Immunohistochemistry of APO-E inhuman liver tissue with APO-E antibody at 2.5 µg/ml.

Immunofluorescence of APO E in mouse brain tissue with APO E Antibody at 20 µg/mL. Green: APO E antibody (orb1239091) Red: Phylloidin staining Blue: DAPI staining.

Immunohistochemistry of APO E in mouse brain tissue with APO E Antibody at 5 µg/mL.