Apolipoprotein E/APOE Antibody

• Catalog Number: orb251510
• Category: Antibodies
• Description: Apolipoprotein E/APOE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317). Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells ELISA (Cap), 1-5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 36154 MW
• UniProt ID: P02649
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Apolipoprotein E;Apo-E;APOE;
• Note: For research use only
• Application notes: WB: The detection limit for Apolipoprotein E is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-Apolipoprotein E antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Apolipoprotein E using anti-Apolipoprotein E antibody.Lane 1:human placenta tissue;2:human HepG2 cell.

IF analysis of Apolipoprotein E using anti-Apolipoprotein E antibody. Apolipoprotein E was detected in a paraffin-embedded section of human placenta tissue.

Sandwich ELISA - Recombinant human Apolipoprotein E/APOE protein standard curve.Use in combination with reagents from human APOE ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody.Apolipoprotein E was detected in paraffin-embedded section of Human Placenta Tissue.