Alpha Actin Antibody / ACTA2 (smooth muscle)

• Catalog Number: orb385893
• Category: Antibodies
• Description: Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SMAT2-1
• Tested applications: IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Smooth Muscle Actin antibody is available for research use only.
• UniProt ID: P62736
• Storage: Store the Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human angiosarcoma using Smooth Muscle Actin antibody

Immunohistochemical staining of Human leiomyosarcoma using Smooth Muscle Actin antibody

Immunohistochemical staining of Rat lung using Smooth Muscle Actin antibody