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Alpha Actin Antibody / ACTA2 (smooth muscle)

Catalog Number: orb385893

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb385893
CategoryAntibodies
DescriptionActins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq]
Species/HostMouse
ClonalityMonoclonal
Clone NumberSMAT2-1
Tested applicationsIF, IHC-P
ReactivityHuman, Rat
IsotypeMouse IgG2a, kappa
ImmunogenRecombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Smooth Muscle Actin antibody is available for research use only.
UniProt IDP62736
StorageStore the Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Alpha Actin Antibody / ACTA2 (smooth muscle)

IHC testing of FFPE human angiosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Alpha Actin Antibody / ACTA2 (smooth muscle)

IHC testing of FFPE human leiomyosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Alpha Actin Antibody / ACTA2 (smooth muscle)

IHC testing of FFPE rat lung with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Alpha Actin Antibody / ACTA2 (smooth muscle)

Immunofluorescent staining of permeabilized human HeLa cells with Smooth Muscle Actin antibody (green, clone SMAT2-1) and Reddot nuclear stain (red).

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    IF,  IHC-P

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    IF,  IHC-P

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
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    FACS,  IF,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • Alpha Actin Antibody / ACTA2 (smooth muscle) [orb2641860]

    IF,  IHC-P

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • Alpha Actin Antibody / ACTA2 (smooth muscle) [orb2637505]

    FACS,  IF,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg