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Alpha Actin Antibody / ACTA2 (smooth muscle)

Catalog Number: orb2637505

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2637505
CategoryAntibodies
DescriptionActin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.
Species/HostMouse
ClonalityMonoclonal
Clone Number1A4
Tested applicationsFACS, IF, IHC-P
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG2a, kappa
ImmunogenAn N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth Muscle Actin antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Smooth Muscle Actin antibody is available for research use only.
Entrez59
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesVariations in protocols, secondaries and substrates may require the Smooth Muscle Actin antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Alpha Actin Antibody / ACTA2 (smooth muscle)

Immunofluorescent staining of fixed human HeLa cells with Smooth Muscle Actin antibody (clone 1A4, green) and NucSpot nuclear stain (red).

Alpha Actin Antibody / ACTA2 (smooth muscle)

IHC: FFPE human leiomyosarcoma stained with Smooth Muscle Actin antibody (clone 1A4).

Alpha Actin Antibody / ACTA2 (smooth muscle)

Flow cytometry testing of fixed human HeLa cells with Smooth Muscle Actin antibody (clone 1A4); Red = isotype control, Blue = Smooth Muscle Actin antibody.

Alpha Actin Antibody / ACTA2 (smooth muscle)

SDS-PAGE analysis of purified, BSA-free Smooth Muscle Actin antibody (clone 1A4) as confirmation of integrity and purity.

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    IF,  IHC-P

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg
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    IF,  IHC-P

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
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    IF,  IHC-P

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • Alpha Actin Antibody / ACTA2 (smooth muscle) [orb749297]

    FACS,  IF,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • Alpha Actin Antibody / ACTA2 (smooth muscle) [orb2641860]

    IF,  IHC-P

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg