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Catalog Number | orb2641860 |
---|---|
Category | Antibodies |
Description | Actins are a family of proteins which are highly conserved and play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. [RefSeq] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SMAT2-1 |
Tested applications | IF, IHC-P |
Reactivity | Human, Rat |
Isotype | Mouse IgG2a, kappa |
Immunogen | Recombinant full-length human ACTA2 protein was used as the immunogen for the Smooth Muscle Actin antibody. |
Antibody Type | Primary Antibody |
Dilution range | Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Smooth Muscle Actin antibody is available for research use only. |
UniProt ID | P62736 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Smooth Muscle Actin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human angiosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human leiomyosarcoma with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat lung with Smooth Muscle Actin antibody (clone SMAT2-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of permeabilized human HeLa cells with Smooth Muscle Actin antibody (green, clone SMAT2-1) and Reddot nuclear stain (red).
IF, IHC-P | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |