Alpha Actin Antibody / ACTA2 (smooth muscle)

• Catalog Number: orb2639148
• Category: Antibodies
• Description: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ACTA2/791
• Tested applications: IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Alpha Smooth Muscle Actin antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Alpha Smooth Muscle Actin antibody is available for research use only.
• UniProt ID: P62736
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Bcl-X antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with alpha Smooth Muscle Actin antibody (clone ACTA2/791).

IHC: Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with alpha Smooth Muscle Actin antibody (clone ACTA2/791).

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with alpha Smooth Muscle Actin antibody (clone ACTA2/791).

IHC: Formalin-fixed, paraffin-embedded rat lung stained with alpha Smooth Muscle Actin antibody (clone ACTA2/791).

Immunofluorescent staining of permeabilized human HeLa cells with Alpha Smooth Muscle Actin antibody (green, clone ACTA2/791) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free Alpha Smooth Muscle Actin antibody antibody (clone ACTA2/791) as confirmation of integrity and purity.