AKT pS473 antibody

• Catalog Number: orb345378
• Category: Antibodies
• Description: AKT pS473 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IF, IHC, Multiplex Assay, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Rabbit Anti-AKTpS473 Antibody was prepared by repeated immunizations in rabbits with a synthetic peptide corresponding to a C-terminus region near phospho Serine 473 of the human, mouse, rat and chicken AKT proteins conjugated to KLH.
• Concentration: 1.15 mg/mL
• Dilution range: ELISA: 1:15,000 - 1:60,000, IHC: 1:100 - 1:500, IF: User Optimized, WB: 1:200 - 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against a non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKTpS473. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
• Conjugation: Unconjugated
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-AKT pS473 Antibody, AKT1 phospho S473,
• Note: For research use only
• Application notes: This AKT antibody is phospho specific for pS473 and is tested in ELISA, western blotting, immunohistochemistry (formalin-fixed paraffin-embedded sections), and immunofluorescence. By immunoblot a single band of the expected apparent molecular weight ~56kDa is observed. For immunohistochemistry no pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of human breast carcinoma tissue using AKT pS473 antibody

Immunohistochemical staining of human breast carcinoma tissue using AKT pS473 antibody

Immunofluorescence analysis of cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required using AKT pS473 antibody

Immunohistochemical staining of human breast carcinoma tissue using AKT pS473 antibody

Western blot analysis of nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase. Load: 35 ug per lane using AKT pS473 antibody