Akt (phospho-S473) antibody (ATTO 594)

• Catalog Number: orb344562
• Category: Antibodies
• Description: Akt (phospho-S473) antibody (ATTO 594)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 17F6.B11
• Tested applications: ELISA, FC, FLISA, IF, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG1
• Immunogen: Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, FLISA: >1:20,000, FC: User Optimized, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
• Conjugation: ATTO-594
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-AKT pS473 ATTO 594 conjugated Antibody,
• Note: For research use only
• Application notes: Anti-AKT pS473 Antibody ATTO 594 Conjugated is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This antibody has been tested in fluorescent western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH 3T3 cells using Akt (phospho-S473) antibody (ATTO 594)

Western blot analysis of GST Tagged AKT 1 Active Recombinant Protein using Akt (phospho-S473) antibody (ATTO 594)

Immunofluorescence analysis of human umbilical vein endothelial cells using Akt (phospho-S473) antibody (ATTO 594)