AKT pS473 antibody (Biotin)

• Catalog Number: orb344541
• Category: Antibodies
• Description: AKT pS473 antibody (Biotin)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 17F6.B11
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG1
• Immunogen: Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
• Concentration: 0.5 mg/mL
• Dilution range: ELISA: 1:20,000, FC: User Optimized, IHC: 20 µg/mL, IF: 1:500-1:3,000, IP: User Optimized, WB: 1:500-1:3,000
• Form/Appearance: Lyophilized
• Purity: Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
• Conjugation: Biotin
• UniProt ID: P31749
• NCBI: 62241011
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-AKT pS473 biotin conjugated Antibody, b
• Note: For research use only
• Application notes: Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is tested for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using AKT pS473 antibody (Biotin)

Immunohistochemical staining of mouse anti AKT phospho S473 biotin conjugated at 40X (left) with negative control (right) Tissue: prostate Fixation using AKT pS473 antibody (Biotin)

Immunohistochemical staining of mouse Anti-AKT pS473 (MOUSE) Biotin Conjugated

Western blot analysis of GST tagged AKT1 active recombinant protein using AKT pS473 antibody (Biotin)

Western blot analysis of GST tagged AKT1 active recombinant protein using AKT pS473 antibody (Biotin)