ACE2 Antibody

• Catalog Number: orb1274388
• Category: Antibodies
• Description: ACE2 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: IHC, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Synthetic Peptide
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACE2
• UniProt ID: Q9BYF1
• NCBI: Q9BYF1
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
• Alternative names: ACEH, Angiotensin-converting enzyme 2, ACE-related
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse and Rat Tissue Lysates. Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, orb1274388, at 1:1000 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.

Western Blot Validation in HepG2 Cells. Loading: 25 µg of lysates per lane. Antibodies: ACE2 antibody, orb1274388, at 1:500 dilution. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue. Immunohistochemistry of paraffin-embedded mouse kidney tissue using ACE2 antibody, orb1274388, at 1:100 dilution (40x lens).