ZO1 antibody

• Catalog Number: orb556551
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZO1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-Fr, IP, WB
• Reactivity: Fish, Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.
• Concentration: 0.4 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 1XPBS, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 195
• Target: ZO1
• UniProt ID: Q07157
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS (pH7), 1% rAlbumin, 20% Glycerol
• Alternative names: tight junction protein 1 , ZO-1
• Note: For research use only
• Application notes: Positive control: HeLa
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of NT2D1 cells using ZO1 antibody

Immunohistochemical staining of H661 xenograft using ZO1 antibody

Immunohistochemical staining of mouse intestine using ZO1 antibody

Immunohistochemical staining of mouse testis using ZO1 antibody

Western blot analysis of Various whole cell using ZO1 antibody

Western blot analysis of Various whole cell using ZO1 antibody

Western blot analysis of Mouse tissue using ZO1 antibody