VTN Antibody

• Catalog Number: orb1263202
• Category: Antibodies
• Description: VTN Antibody
• Target: VTN
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the N-terminal region of human VTN.
• UniProt ID: P04004
• MW: 54 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:8000For IHC-P starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Vitronectin, VN, S-protein, Serum-spreading factor
• Note: For research use only
• NCBI: P04004

Overlay histogram showing MCF-7 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:32000 dilution + human plasma lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:8000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with VTN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.