UCHL1 Antibody

• Catalog Number: orb1252686
• Category: Antibodies
• Description: UCHL1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 31A3
• Tested applications: IHC, WB
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Isotype: IgG1, kappa
• Immunogen: Native protein from brain was used as the immunogen for this PGP9.5 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UCHL1
• UniProt ID: P09936
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Ubiquitin carboxyl-terminal hydrolase isozyme L1,
• Note: For research use only
• Application notes: WB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of human brain with PGP9.5 antibody (31A3).

Western blot of human, mouse and rat brain lysate using PGP9.5 antibody (31A3).