UCHL1 Antibody

• Catalog Number: orb1252015
• Category: Antibodies
• Description: UCHL1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UBCE-L1
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the PGP9.5 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the PGP9.5 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UCHL1
• UniProt ID: P09936
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Ubiquitin carboxyl-terminal hydrolase isozyme L1,
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the PGP9.5 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE rat cerebellum with PGP9.5 antibody (clone UBCE-L1).