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Catalog Number | orb28553 |
---|---|
Category | Antibodies |
Description | Purified rabbit polyclonal antibody. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB2803 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Rat, Zebrafish |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Immunogen | Synthetic Peptide |
Dilution range | WB: 1:1000, IHC-P: 1:50-100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 51852 |
Target | UBA3 |
UniProt ID | Q8TBC4 |
NCBI | NP_937838.1, NP_003959.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Anti-NEDD8-activating enzyme E1 catalytic subunit Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
UBE1C Antibody (S430) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).
Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C Antibody (S430). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-UBE1 C-term Pab is used in Western blot to detect UBE1 in mouse brain tissue lysate.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Rat, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |