UBA3 Antibody

• Catalog Number: orb1270700
• Category: Antibodies
• Description: UBA3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rat, Zebrafish
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 amino acids from the C-terminal region of human UBE1C.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: UBA3
• UniProt ID: Q8TBC4
• NCBI: Q8TBC4
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: NEDD8-activating enzyme E1 catalytic subunit, 632-
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Antibody is used in Western blot to detect UBE1 in mouse brain tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis in HL-60 cell line lysates (35 ug/lane).

Western blot analysis of UBE1C using rabbit polyclonal UBE1C Antibody (S430) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.