UBA3 antibody

• Catalog Number: orb668550
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBA3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human UBA3. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: UBA3
• Entrez: 117553, 22200, 9039
• UniProt ID: Q8TBC4, Q99MI7, Q8C878
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-UBE1C antibody, anti-NEDD8-activating enzyme
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of UBA3 expression in HepG2 (A), Jurkat (B), mouse heart (C), rat brain (D) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 53 kD)

Immunohistochemical analysis of UBA3 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.