TUBB3 Antibody

• Catalog Number: orb1264082
• Category: Antibodies
• Description: TUBB3 Antibody
• Target: TUBB3
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus, Monkey, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This TUBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human TUBB3.
• UniProt ID: Q13509
• MW: 50 kDa
• Tested applications: FC, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tubulin beta-3 chain, Tubulin beta-4 chain, Tubuli
• Note: For research use only
• NCBI: Q13509

Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in mouse brain tissue lysates (15 ug/lane).

Western blot analysis in HepG2 cell line lysates (15 ug/lane).

TUBB3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.