TUBB3 Antibody

• Catalog Number: orb1264082
• Category: Antibodies
• Description: TUBB3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Monkey, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This TUBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human TUBB3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: TUBB3
• UniProt ID: Q13509
• NCBI: Q13509
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Tubulin beta-3 chain, Tubulin beta-4 chain, Tubuli
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in mouse brain tissue lysates (15 ug/lane).

Western blot analysis in HepG2 cell line lysates (15 ug/lane).

TUBB3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.