Topoisomerase 2 alpha antibody

• Catalog Number: orb705544
• Category: Antibodies
• Description: Mouse monoclonal antibody to Topoisomerase 2 alpha
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Topoisomerase 2 alpha. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300, IF: 1:100-1:500
• Form/Appearance: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: TOP2A
• Entrez: 7153
• UniProt ID: P11388
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Alternative names: Anti-TOP2 antibody, anti-DNA topoisomerase 2-alpha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Topoisomerase 2 alpha expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates. (Predicted band size: 174 kD; Observed band size: 174 kD)

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.