TOP2A Antibody

• Catalog Number: orb1262303
• Category: Antibodies
• Description: TOP2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Hamster, Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1167-1196 amino acids from the C-terminal region of human TOP2A.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 174 kDa
• Target: TOP2A
• UniProt ID: P11388
• NCBI: P11388
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: DNA topoisomerase 2-alpha, DNA topoisomerase II, a
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of TOP2A Antibody in A375 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Confocal immunofluorescent analysis of TOP2A Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).