Topoisomerase II alpha/TOP2A Antibody

• Catalog Number: orb570416
• Category: Antibodies
• Description: Topoisomerase II alpha/TOP2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence of human Topoisomerase II alpha/TOP2A (EDYLYGQTTTYLTYND).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 174 kDa
• UniProt ID: P11388
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: DNA topoisomerase 2-alpha; DNA topoisomerase II, a
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IF analysis of TOP2A using anti-TOP2A antibody.

WB analysis using anti-TOP2A antibody.Lane 1:A549 cell, Lane 2:U2OS cell, Lane 3:HEK293 cell, Lane 4:K562 cell.

Flow Cytometry analysis of 293T cells using anti-TOP2A antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IHC analysis of TOP2A using anti-TOP2A antibody.

IHC analysis of TOP2A using anti-TOP2A antibody.