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Catalog Number | orb1252176 |
---|---|
Category | Antibodies |
Description | TOP2A Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | TOP2A/1362 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG2b, kappa |
Immunogen | Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlWestern blot: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TOP2A |
UniProt ID | P11388 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | DNA topoisomerase 2-alpha, DNA topoisomerase II, a Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlWestern blot: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human tonsil with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human bladder carcinoma with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Monkey | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Hamster, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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