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Catalog Number | orb1272291 |
---|---|
Category | Antibodies |
Description | TNF Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTNF-alpha (human Tumor Necrosis Factor-alpha). |
Concentration | batch dependent |
Dilution range | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-α (0.25 ng/mL), a concentration of 0.012 - 0.014 μg/mL of this antibody is required. ELISA:To detect hTNF-α by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTNF-α. Sandwich:To detect hTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TNF-αas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α.Western Blot:To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | TNF |
UniProt ID | P01375 |
NCBI | P01375 |
Storage | TNF-a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis facto Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human TNF-alpha (orb1272290) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 ug/ml-1.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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